Robo3^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Robo3^tm1Matl
• Name: roundabout guidance receptor 3; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:3043150
• Synonyms: Rig-1-, Robo3^GFP
• Gene: Robo3 Location: Chr9:37327341-37344730 bp, - strand Genetic Position: Chr9, 20.76 cM, cytoband B
• Alliance: Robo3^tm1Matl page

Reduced internuclear commissure in Robo3^tm1.1Ache/Robo3^tm1.1Ache Egr2^tm2(cre)Pch/Egr2⁺ and Robo3^tm1Matl/Robo3^tm1Matl mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89438
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A portion of exon 1 including the ATG start site was replaced with a cassette containing an IRES element, a tau-GFP fusion gene, a loxP site, a PGK-1 promoter, a neomycin resitance gene, a PGK-1 polyA tail and a second loxP site. Western blot analysis confirmed the absence of protein in spinal cords from homozygous null mice. The tau-GFP fusion protein is expressed from the Robo3 promoter. (J:89438)

Expression

In Mice Carrying this Mutation:	40 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Robo3 Mutation: 76 strains or lines available

References

• Original: J:89438 Sabatier C, et al., The divergent Robo family protein rig-1/Robo3 is a negative regulator of slit responsiveness required for midline crossing by commissural axons. Cell. 2004 Apr 16;117(2):157-69
• All: 25 reference(s)