Scn8a^tm1Mm
Targeted Allele Detail

Summary

• Symbol: Scn8a^tm1Mm
• Name: sodium channel, voltage-gated, type VIII, alpha; targeted mutation 1, Miriam Meisler
• MGI ID: MGI:3043395
• Synonyms: Scn8a^flox
• Gene: Scn8a Location: Chr15:100767739-100943819 bp, + strand Genetic Position: Chr15, 56.39 cM, cytoband F1
• Alliance: Scn8a^tm1Mm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91696
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites have been inserted 107 bp upstream and 134 bp downstream flanking exon 1, the first coding exon. An frt flanked neomycin resistance cassette was inserted downstream of of exon 1 as well. Mice were bred to transgenic mice expressing Flp recombinase to excise the neomycin cassette in the germ line. The resulting mice containing a floxed exon 1 were bred to homozygosity. Normal protein levels were detected by Western blot analysis. (J:91696)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scn8a Mutation: 99 strains or lines available

References

• Original: J:91696 Levin SI, et al., Floxed allele for conditional inactivation of the voltage-gated sodium channel Scn8a (Na(v)1.6). Genesis. 2004 Aug;39(4):234-9
• All: 10 reference(s)