Pdpk1^tm2Ejm
Targeted Allele Detail

Summary

• Symbol: Pdpk1^tm2Ejm
• Name: 3-phosphoinositide dependent protein kinase 1; targeted mutation 2, Edward J McManus
• MGI ID: MGI:3043785
• Synonyms: PDK1(155E)
• Gene: Pdpk1 Location: Chr17:24292654-24369898 bp, - strand Genetic Position: Chr17, 12.23 cM, cytoband A3.3
• Alliance: Pdpk1^tm2Ejm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90081
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Other
• Mutation details: In order to disrupt the PIF pocket, the codon in exon 4 encoding leucin 144 was engineered to encode glutamic acid. (J:85056, J:90081)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdpk1 Mutation: 138 strains or lines available

References

• Original: J:90081 McManus EJ, et al., The in vivo role of PtdIns(3,4,5)P(3) binding to PDK1 PH domain defined by knockin mutation. EMBO J. 2004 May 19;23(10):2071-82
• All: 8 reference(s)