Ppp3r1^tm1Grc
Targeted Allele Detail

Summary

• Symbol: Ppp3r1^tm1Grc
• Name: protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); targeted mutation 1, Gerald R Crabtree
• MGI ID: MGI:3043986
• Synonyms: CnB^*
• Gene: Ppp3r1 Location: Chr11:17109298-17150380 bp, + strand Genetic Position: Chr11, 9.71 cM, cytoband A3.1
• Alliance: Ppp3r1^tm1Grc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70266
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Other
• Mutation details: Sequence encoding an FRB^* peptide tag was inserted into exon 5, immediately following the last codon of the coding sequence. This allele produces a protein with a C-terminal FRB^* peptide tag that prevents interaction with PPP3CA, thereby eliminating calcium-dependent activation of PPP3CA catalytic activity. An assay indicated the abrogation of caclcineurin phosphatase activity. (J:70266)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp3r1 Mutation: 30 strains or lines available

References

• Original: J:70266 Graef IA, et al., Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Cell. 2001 Jun 29;105(7):863-75
• All: 1 reference(s)