App^tm1Ini
Targeted Allele Detail

Summary

• Symbol: App^tm1Ini
• Name: amyloid beta precursor protein; targeted mutation 1, Ikuo Nishimoto
• MGI ID: MGI:3044176
• Synonyms: V642I-APP-KI
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm1Ini page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90296
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Insertion
• Mutation details: A point mutation was introduced into exon 17 causing a valine to isoleucine change at amino acid residue 642 (V642I) together with a floxed neomycin resistance gene in intron 17. The neomycin gene was subsequently excised from correctly targeted clones by transient cre expression. The mutation was confirmed in mice by genomic DNA sequencing. (J:90296, J:90561)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any App Mutation: 105 strains or lines available

References

• Original: J:90296 Kawasumi M, et al., Targeted introduction of V642I mutation in amyloid precursor protein gene causes functional abnormality resembling early stage of Alzheimer's disease in aged mice. Eur J Neurosci. 2004 May;19(10):2826-38
• All: 2 reference(s)