Tcf7l1^tm2Efu
Targeted Allele Detail

Summary

• Symbol: Tcf7l1^tm2Efu
• Name: transcription factor 7 like 1 (T cell specific, HMG box); targeted mutation 2, Elaine Fuchs
• MGI ID: MGI:3044616
• Synonyms: Tcf3-
• Gene: Tcf7l1 Location: Chr6:72603354-72766028 bp, - strand Genetic Position: Chr6, 32.27 cM
• Alliance: Tcf7l1^tm2Efu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90402
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP flanked 64 bp segment of exon 2 and a neo cassette were inserted via homologous recombination then removed via cre mediated recombination prior to generation of chimeric mice. The resulting allele lacked exon 2 and contained a frameshift and early termination codon. Immunoblot analysis confirmed the absence of protein expression in homozygous embryos (J:90402)

Expression

In Mice Carrying this Mutation:	72 assay results
In Structures Affected by this Mutation:	10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcf7l1 Mutation: 84 strains or lines available

References

• Original: J:90402 Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
• All: 4 reference(s)