Spi1^tm1Kast
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1Kast
• Name: Spi-1 proto-oncogene; targeted mutation 1, Philippe Kastner
• MGI ID: MGI:3044765
• Synonyms: PU.1^G, PU-1-GFP
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1Kast page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90543
• Parent Cell Line: P1 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 22 bp segment of exon 1 was replaced with a rabbit beta globin intron, an enhanced GFP sequence and an SV40 polyadenylation signal via homologous recombination. Both mRNA and protein were undetectable in fetal liver from homozygous mice. GFP expression correlated well with endogenous Sfpi1 expression. (J:90543)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

References

• Original: J:90543 Back J, et al., PU.1 determines the self-renewal capacity of erythroid progenitor cells. Blood. 2004 May 15;103(10):3615-23
• All: 10 reference(s)