Mfge8^tm1Osa
Targeted Allele Detail

Summary

• Symbol: Mfge8^tm1Osa
• Name: milk fat globule EGF and factor V/VIII domain containing; targeted mutation 1, Shigekazu Nagata
• MGI ID: MGI:3044898
• Synonyms: MFG-E8^-
• Gene: Mfge8 Location: Chr7:78783516-78798808 bp, - strand Genetic Position: Chr7, 44.9 cM
• Alliance: Mfge8^tm1Osa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90633
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomysin resistance cassette replaced exons 4-6. Southern blot confirmed recombination in mutant tails. Western blot analysis showed no protein expression in macrophages. (J:90633)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mfge8 Mutation: 23 strains or lines available

References

• Original: J:90633 Hanayama R, et al., Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice. Science. 2004 May 21;304(5674):1147-50
• All: 40 reference(s)