Tert^tm1Leah
Targeted Allele Detail

Summary

• Symbol: Tert^tm1Leah
• Name: telomerase reverse transcriptase; targeted mutation 1, Lea Harrington
• MGI ID: MGI:3044918
• Synonyms: mTert^-, Tert^delta
• Gene: Tert Location: Chr13:73775030-73797962 bp, + strand Genetic Position: Chr13, 40.12 cM
• Alliance: Tert^tm1Leah page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90866
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Five exons were replaced with a neomycin selection cassette inserted by homologous recombination. The deletion was reported to disrupt the telomerase-specific motif as well as the first three of the seven reverse transcriptase motifs. Transcript was undetected in homozygous mutant ES cells and in testicular tissue from first generation homozygous mutant mice, as shown by RT-PCR analysis. No telomerase activity was detected in homozygous mutant ES cell clones and in tissue extracts from homozygous mutant testes, liver, thymus, lung and kidney, as determined by TRAP analysis. (J:90866)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tert Mutation: 55 strains or lines available

References

• Original: J:90866 Liu Y, et al., The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo. Curr Biol. 2000 Nov 16;10(22):1459-62
• All: 24 reference(s)