Col2a1^tm1(SOX9)Crm
Targeted Allele Detail

Summary

• Symbol: Col2a1^tm1(SOX9)Crm
• Name: collagen, type II, alpha 1; targeted mutation 1, Benoit de Crombrugghe
• MGI ID: MGI:3045169
• Gene: Col2a1 Location: Chr15:97873483-97902525 bp, - strand Genetic Position: Chr15, 53.97 cM
• Alliance: Col2a1^tm1(SOX9)Crm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90567
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A 3xHA-tagged human SOX9 cDNA sequence and floxed neo were inserted upstream of exon 2 by homologous recombination. Transient cre-mediated recombination excised the neo, leaving only the human SOX9 insertion sequence. Northern blot of rib cartilage in heterozygous mutant mice revealed knocked-in SOX9 gene expression was ~20% of that of endogenous Sox9 gene expression. (J:90567)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col2a1 Mutation: 69 strains or lines available

References

• Original: J:90567 Akiyama H, et al., Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev. 2004 May 1;18(9):1072-87
• All: 2 reference(s)