Spi1^tm1.1Dgt
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1.1Dgt
• Name: Spi-1 proto-oncogene; targeted mutation 1.1, Daniel G Tenen
• MGI ID: MGI:3045207
• Synonyms: deltaUREneo, (frt-PGKneo-frt----loxp), PU.1^kd/kd, PU.1-knockdown, Sfpi1^kd/kd, Sfpi1-knockdown, UREdeltaneo
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1.1Dgt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90331
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Intragenic deletion
• Mutation details: Sfpi1^tm1Dgt mice were crossed with mice carrying Tg(CMV-cre)1Cgn. Activity of the cre recombinase resulted in excision of the upstream regulatory element producing a hypomorphic allele. Mice homozygous for this allele produced only about 20% of wild-type protein product. (J:90331)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

References

• Original: J:90331 Rosenbauer F, et al., Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU.1. Nat Genet. 2004 Jun;36(6):624-30
• All: 6 reference(s)