Smad1^tm2Sor
Targeted Allele Detail

Summary

• Symbol: Smad1^tm2Sor
• Name: SMAD family member 1; targeted mutation 2, Philippe Soriano
• MGI ID: MGI:3045219
• Synonyms: Smad1^L
• Gene: Smad1 Location: Chr8:80065024-80126057 bp, - strand Genetic Position: Chr8, 37.56 cM
• Alliance: Smad1^tm2Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90769
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Homologous recombination incorporated serine to alanine substitutions in the MAPK-consensus phosphorylation sites (p.S187A, p.S195A, p.S206A, p.S214A) and two conserved phosphoserines in exon 3 (p.(S209_S210delinsAA)). Western blot analysis of MEF nuclear extracts demonstrated the mutant protein was expressed at similar levels as that of wild-type. (J:90769)

Expression

In Mice Carrying this Mutation:	39 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smad1 Mutation: 32 strains or lines available

References

• Original: J:90769 Aubin J, et al., In vivo convergence of BMP and MAPK signaling pathways: impact of differential Smad1 phosphorylation on development and homeostasis. Genes Dev. 2004 Jun 15;18(12):1482-94
• All: 2 reference(s)