Slc5a7^tm1Rbl
Targeted Allele Detail

Summary

• Symbol: Slc5a7^tm1Rbl
• Name: solute carrier family 5 (choline transporter), member 7; targeted mutation 1, Randy D Blakely
• MGI ID: MGI:3045463
• Synonyms: CHT^-
• Gene: Slc5a7 Location: Chr17:54580618-54606062 bp, - strand Genetic Position: Chr17, 28.23 cM, cytoband D
• Alliance: Slc5a7^tm1Rbl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90846
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: EGFP and Neo cassettes replaced part of the coding region beginning at the ATG in exon two and extending into intron 4. LoxP sites flanked the EGFP and FRT sites flanked the neo. Southern blot and PCR confirmed recombination in mutants and western blot indicated a reduction of protein levels in heterozygous mice and the absence of protein immunoactivity in the homozygous mutants. (J:90846)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc5a7 Mutation: 42 strains or lines available

References

• Original: J:90846 Ferguson SM, et al., Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice. Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8762-7
• All: 7 reference(s)