Ncilt^C57BL/10J
QTL Variant Detail

Summary

• QTL variant: Ncilt^C57BL/10J
• Name: nuclear cytoplasmic invaginations in lung tumors; C57BL/10J
• MGI ID: MGI:3045607
• Synonyms: Ncilt^B10.O20
• QTL: Ncilt Location: Chr6:145162425-145195965 bp Genetic Position: Chr6, Syntenic

Variant origin

• Strain of Specimen: C57BL/10J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: This allele confers decreased incidence of nuclear cytoplasmic invaginations in lung tumors compared to O20/A. (J:68464)

Notes

Homozygosity for B10.O20-derived alleles at this locus is associated with the absence of nuclear cytoplasmic invaginations (NCIs) in lung tumors.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:68464

Linkage analysis was performed on F2 crosses derived between 3 different OcB/Dem recombinant congenic strains (OcB-4/Dem, OcB-6/Dem, OcB-9/Dem) and O20/A to identify QTLs associated with susceptibility to lung tumors. (The OcB/Dem RC strains are derived from progenitor strains O20/A and B10.O20.) Parental strain O20/A is susceptible to lung tumors whereas parental strain B10.O20 is resistant. F2 animals were treated with ENU in utero and lung tumors were assessed for nuclear cytoplasmic invaginations (NCI) and lymphocytic infiltration (LI).

Linkage to NCI was observed in the (OcB-6/Dem x O20/A)F2 cross at the Kras locus (P=0.0456) at 71 cM on mouse Chromosome 6. This locus is named Ncilt (nuclear cytoplasmic invaginations in lung tumors.) Inheritance of 2 B10.O20-derived alleles at Kras is strongly correlated with the presence of NCI in lung tumors whereas inheritance of 2 O20/A-derived alleles is strongly correlated with the absence of NCI in lung tumors. In this cross, the presence of NCIs is seen insmaller tumors whereas the presence of LI is seen in larger tumors.

References

• Original: J:68464 Tripodis N, et al., Genetic linkage of nuclear morphology of mouse lung tumors to the Kras-2 locus. Exp Lung Res. 2001 Apr-May;27(3):185-96
• All: 1 reference(s)