Igh-J^{tm1(VDJ-17.2.25)Wabl}
Targeted Allele Detail

Summary

• Symbol: Igh-J^{tm1(VDJ-17.2.25)Wabl}
• Name: immunoglobulin heavy chain, joining region; targeted mutation 1, Matthias Wabl
• MGI ID: MGI:3045610
• Synonyms: VHT
• Gene: Igh-J Location: unknown Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33628
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: All four heavy chain J elements were replaced with a neo and a rearranged heavy chain VDJ region for an antibody specific for hapten (4-hydroxy-3-nitrophenyl) acetyl (NP). Southern blot confirmed recombination. Flow cytometry confirmed expression of the inserted VDJ region. (J:33628)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cx1	Igh-J^tm1Dhu/Igh-J^tm1(VDJ-17.2.25)Wabl Igk^tm1Dhu/Igk^tm1Dhu	Not Specified

Phenotypes:

Affected Systems	cx1
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hematopoietic system	√
abnormal B cell differentiation	√
abnormal immature B cell morphology	√
immune system	√
abnormal B cell differentiation	√
abnormal immature B cell morphology	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh-J Mutation: 13 strains or lines available

References

• Original: J:33628 Cascalho M, et al., A quasi-monoclonal mouse [see comments]. Science. 1996 Jun 14;272(5268):1649-52
• All: 27 reference(s)