Rr27^tm2Rohl
Targeted Allele Detail

Summary

• Symbol: Rr27^tm2Rohl
• Name: regulatory region 27; targeted mutation 2, Rolf Ohlsson
• MGI ID: MGI:3045724
• Synonyms: H19^tm2Rohl
• Gene: Rr27 Location: Chr7:142133885-142136283 bp Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm2Rohl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89915
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: CTCF target site 4 within the imprinting control region (ICR) was modified from a central GTGG motif to ATAT via homologous recombination. A floxed neomycin resistance gene cassette that was included in the targeting vector was excised via in vivo cre-mediated recombination in the germ line. Southern blot analysis and sequence analysis confirmed recombination. (J:89915)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:89915 Pant V, et al., Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance. Mol Cell Biol. 2004 Apr;24(8):3497-504
• All: 1 reference(s)