Sfrp1^tm1Bsk
Targeted Allele Detail

Summary

• Symbol: Sfrp1^tm1Bsk
• Name: secreted frizzled-related protein 1; targeted mutation 1, Barry S Komm
• MGI ID: MGI:3045799
• Synonyms: sFRP-1^-
• Gene: Sfrp1 Location: Chr8:23901518-23939648 bp, + strand Genetic Position: Chr8, 11.48 cM
• Alliance: Sfrp1^tm1Bsk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89547
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1, which encodes most of the protein and the entire CRD, was replaced by a LacZ/MC1-neo cassette. PCR analysis demonstrated that homozygous mutants contained the LacZ gene. Northern blot of kidneys indicated no presence of transcript for the CRD domain of the endogenous gene. RT-PCR analysis of calvaria showed lack of expression of both the CRD (the 5' region of the transcript) and the netrin domain (the 3' region) in mutants. (J:89547)

Expression

In Mice Carrying this Mutation:	85 assay results
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sfrp1 Mutation: 19 strains or lines available

References

• Original: J:89547 Bodine PV, et al., The Wnt antagonist secreted frizzled-related protein-1 is a negative regulator of trabecular bone formation in adult mice. Mol Endocrinol. 2004 May;18(5):1222-37
• All: 6 reference(s)