Kit^tm2Ber
Targeted Allele Detail

Summary

• Symbol: Kit^tm2Ber
• Name: KIT proto-oncogene receptor tyrosine kinase; targeted mutation 2, Alan Bernstein
• MGI ID: MGI:3045802
• Synonyms: Kit^569F
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^tm2Ber page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89602
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A tyrosine to phenylalanine mutation (Y569F) was introduced into the juxtamembrane (Jx) domain in exon 11. Transient cre-mediated recombination removed an intronic floxed neo cassette located downstream of exon 11. Southern blot analysis demonstrated successful recombination. (J:89602)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 182 strains or lines available

References

• Original: J:89602 Kimura Y, et al., Targeted mutations of the juxtamembrane tyrosines in the Kit receptor tyrosine kinase selectively affect multiple cell lineages. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6015-20
• All: 2 reference(s)