Ddx25^tm1Mld
Targeted Allele Detail

Summary

• Symbol: Ddx25^tm1Mld
• Name: DEAD box helicase 25; targeted mutation 1, Maria L Dufau
• MGI ID: MGI:3045804
• Synonyms: GRTH^-
• Gene: Ddx25 Location: Chr9:35453144-35469766 bp, - strand Genetic Position: Chr9, 20.31 cM, cytoband A3-A5
• Alliance: Ddx25^tm1Mld page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89546
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele contains a mutation in the second ATG codon in exon 5, partial deletion of exon 5, and complete deletion of exons 6-7, which contain the conserved ATPase motif, G-GKT, and the PTRELA motif of the RNA helicase family. PCR analysis confirmed recombination and Northern and Western blot indicated lack of transcript and protein in mutant testes. (J:89546)

Expression

In Mice Carrying this Mutation:	5 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ddx25 Mutation: 26 strains or lines available

References

• Original: J:89546 Tsai-Morris CH, et al., Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is essential for spermatid development and completion of spermatogenesis. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6373-8
• All: 7 reference(s)