Rasgrf1^tm1Sva
Targeted Allele Detail

Summary

• Symbol: Rasgrf1^tm1Sva
• Name: RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation 1, Alcino J Silva
• MGI ID: MGI:3045890
• Synonyms: ras-grf1(-)
• Gene: Rasgrf1 Location: Chr9:89791961-89909030 bp, + strand Genetic Position: Chr9, 47.31 cM
• Alliance: Rasgrf1^tm1Sva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91065
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin gene was inserted into an exon encoding part of the Dbl homology domain. Southern blot confirmed recombination and Western blot from adult brain indicated that wild-type protein could not be detected in mutants. (J:91065)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasgrf1 Mutation: 66 strains or lines available

References

• Original: J:91065 Giese KP, et al., Hippocampus-dependent learning and memory is impaired in mice lacking the Ras-guanine-nucleotide releasing factor 1 (Ras-GRF1). Neuropharmacology. 2001 Nov;41(6):791-800
• All: 6 reference(s)