Chat^tm1.1Jrs
Targeted Allele Detail

Summary

• Symbol: Chat^tm1.1Jrs
• Name: choline O-acetyltransferase; targeted mutation 1.1, Joshua R Sanes
• MGI ID: MGI:3045900
• Synonyms: ChAT^-
• Gene: Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
• Alliance: Chat^tm1.1Jrs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91066
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Chat^tm1Jrs-carring mice were crossed with transgenic mice expressing cre in the germ line to produce this allele in which exons 3 and 4 were replaced with an frt-flanked neo. Southern blot confirmed excision of the exons. RT-PCR revealed the splicing of exon 2 to exon 5, resulting in a frame shift after the codon for amino acid 22 and a stop codon at amino acid 31. Therefore, the protein products were less than 32 amino acids long, instead of the 641, and lacked the catalytic center. (J:91066)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chat Mutation: 58 strains or lines available

References

• Original: J:91066 Misgeld T, et al., Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase. Neuron. 2002 Nov 14;36(4):635-48
• All: 4 reference(s)