Rspo2^ftls
Transgenic Allele Detail

Summary

• Symbol: Rspo2^ftls
• Name: R-spondin 2; footless
• MGI ID: MGI:3045965
• Synonyms: ftl, Rspo2^Tg
• Gene: Rspo2 Location: Chr15:42884190-43034222 bp, - strand Genetic Position: Chr15, 16.73 cM
• Alliance: Rspo2^ftls page

Rspo2^ftls/Rspo2^ftls Rspo3^tm1.1Jcob/Rspo3^tm1.2Jcob Tg(Prrx1-cre)1Cjt/0 double mutants have more severe limb defects than single mutants

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic
• Mutations: Insertion, Intragenic deletion
• Mutation details: This mutation was caused by the insertion of 6 to 8 copies of a transgenic construct containing the chloramphenicol acetyltransferase (CAT) gene adjoined to a rat H+/K+ ATPase promoter. Approximately 7 kb of genomic DNA was deleted at the insertion site. This insertion/deletion was shown to be located in intron 3 of the Rspo2 gene. No exons of the gene were deleted. Analysis of the transcript by RT-PCR, northern and in situ hybridization analysis showed that a smaller transcript is expressed from this allele that results from the splicing of exon 3 to a cryptic splice site in the inserted transgene. RT-PCR analysis indicates that a very low level of full-length transcript is expressed; however, no full length transcript is detectable by in-situ hybridization. This allele is described as a severe hypomorph. (J:83881, J:131960)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rspo2 Mutation: 20 strains or lines available

References

• Original: J:83881 Bell SM, et al., Asymmetric limb malformations in a new transgene insertional mutant, footless. Mech Dev. 2003 May;120(5):597-605
• All: 4 reference(s)