Tg(CAG-Bgeo/GFP)21Lbe
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo/GFP)21Lbe
• Name: transgene insertion 21, Corrinne Lobe
• MGI ID: MGI:3046177
• Synonyms: [ACTBBgeo/GFP] 21Lbe, pCaggs-lacZ-GFP, Tg(ACTB-Bgeo/GFP)21Lbe, Z/EG, ZEG, Z/EGLobe21
• Transgene: Tg(CAG-Bgeo/GFP)21Lbe Location: unknown Genetic Position: Chr5, Syntenic
• Alliance: Tg(CAG-Bgeo/GFP)21Lbe page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:83045
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Transgene description

• Transgene Type: Transgenic (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. Upon exposure to cre recombinase to excise the lacZ-polyA cassette, EGFP is expressed in cre dependant manner. This transgene was inserted in the G1 band of Chromosome 5 at approximately 136.35 MB (build 37). (J:83045, J:158413)

Expression

In Mice Carrying this Mutation:

10 assay results 2 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(CAG-Bgeo/GFP)21Lbe

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Expression of lacZ is widespread in transgenic mice, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages.

References

• Original: J:83045 Novak A, et al., Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision. Genesis. 2000 Nov-Dec;28(3-4):147-55
• All: 435 reference(s)