Igkj1^tm1Mss
Targeted Allele Detail

Summary

• Symbol: Igkj1^tm1Mss
• Name: immunoglobulin kappa joining 1; targeted mutation 1, Mark S Schlissel
• MGI ID: MGI:3046652
• Synonyms: kappa^oGFP
• Gene: Igkj1 Location: Chr6:70699546-70699583 bp, + strand Genetic Position: Chr6, 32.04 cM
• Alliance: Igkj1^tm1Mss page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91295
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A promoterless GFP reporter followed by an intron and SV40 polyadenylation signal were inserted into the coding region via homologous recombination. GFP expression is seen in about 10% of B cells. Allele-specific PCR confirmed that insertion of GFP did not alter the rearrangement frequency of this allele compared to the wild-type allele. (J:91295)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igkj1 Mutation: 2 strains or lines available

References

• Original: J:91295 Liang HE, et al., Variegated transcriptional activation of the immunoglobulin kappa locus in pre-b cells contributes to the allelic exclusion of light-chain expression. Cell. 2004 Jul 9;118(1):19-29
• All: 3 reference(s)