Rr27^tm1Pes
Targeted Allele Detail

Summary

• Symbol: Rr27^tm1Pes
• Name: regulatory region 27; targeted mutation 1, Piroska E Szabo
• MGI ID: MGI:3046753
• Synonyms: H19^tm1Pes, H19^tm1Pes-mat, H19^tm1Pes-pat, ICR^delta
• Gene: Rr27 Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Rr27^tm1Pes page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91020
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Insertion
• Mutation details: Point mutations were introduced into the core sequence of all 4 CTCF binding sites resulting in five G-to-T, two C-to-A, one (G/A)-to-T, and one-(T/C)-to-G conversions at each site. A single loxP site was also present after excision of a floxed neomycin resistance gene cassette. EMSA analysis confirmed that these mutated CTCF binding sites are unable to bind CTCF. (J:91020)

Expression

In Mice Carrying this Mutation:	16 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr27 Mutation: 7 strains or lines available

References

• Original: J:91020 Szabo PE, et al., Role of CTCF binding sites in the Igf2/H19 imprinting control region. Mol Cell Biol. 2004 Jun;24(11):4791-800
• All: 5 reference(s)