Polg^tm1.1Lrsn
Targeted Allele Detail

Summary

• Symbol: Polg^tm1.1Lrsn
• Name: polymerase (DNA directed), gamma; targeted mutation 1.1, Nils-Goran Larsson
• MGI ID: MGI:3046825
• Synonyms: mtDNA mutator, Pol^D257A, PolgA^mut
• Gene: Polg Location: Chr7:79095979-79116110 bp, - strand Genetic Position: Chr7, 45.04 cM, cytoband E
• Alliance: Polg^tm1.1Lrsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91077
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Polg^tm1Lrsn mice were mated with ubiquitously expressing Flp-mice to excise the Frt-flanked neo, leaving exon 3 flanked with loxP sites and an aspartic acid to alanine codon change at 257, in the second exonuclease domain. Biochemical assays revealed a profound reduction in exonuclease activity in these mutants. (J:91077)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Polg Mutation: 58 strains or lines available

References

• Original: J:91077 Trifunovic A, et al., Premature ageing in mice expressing defective mitochondrial DNA polymerase. Nature. 2004 May 27;429(6990):417-23
• All: 36 reference(s)