Fmn1^tm1Zllr
Targeted Allele Detail

Summary

• Symbol: Fmn1^tm1Zllr
• Name: formin 1; targeted mutation1, Rolf Zeller
• MGI ID: MGI:3046836
• Synonyms: Fmn^delta10
• Gene: Fmn1 Location: Chr2:113158081-113547112 bp, + strand Genetic Position: Chr2, 57.3 cM, cytoband C1-qter
• Alliance: Fmn1^tm1Zllr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91385
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 10 was completely replaced with a PGK promoter and neomycin resistance gene cassette and a loxP site was inserted further downstream in intron 10. A correctly targeted ES cell line was further modified by the insertion of a loxP site flanked PGK promoter and hygromycin resistance gene cassette and an En-2 splice acceptor-IRES-LacZ expression cassette into exon 24. Removal of exon 10 causes a frame shift mutation, which disrupts the highly conserved formin homology domain SH2 and results in a protein product that includes out-of-frame translation after exon 9. (J:91385)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fmn1 Mutation: 76 strains or lines available

References

• Original: J:91385 Zuniga A, et al., Mouse limb deformity mutations disrupt a global control region within the large regulatory landscape required for Gremlin expression. Genes Dev. 2004 Jul 1;18(13):1553-64
• All: 1 reference(s)