Otx2^tm6Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm6Sia
• Name: orthodenticle homeobox 2; targeted mutation 6, Shinichi Aizawa
• MGI ID: MGI:3046840
• Synonyms: Otx2^deltaFM2
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm6Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91011
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The FM2 enhancer region (1.0 kb) was replaced with a floxed neomycin-resistance gene. Southern blot and PCR confirmed recombination. (J:91011)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Otx2^tm6Sia/Otx2^tm6Sia	involves: C57BL/6NCrlj * CBA/JNCrlj
ht2	Otx2^tm1Sia/Otx2^tm6Sia	involves: C57BL/6NCrlj * CBA/JNCrlj

Phenotypes:

Affected Systems	hm1	ht2
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nervous system	N	√
nervous system phenotype	N
abnormal brain development		√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

6 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:91011 Kurokawa D, et al., Regulation of Otx2 expression and its functions in mouse forebrain and midbrain. Development. 2004 Jul;131(14):3319-31
• All: 2 reference(s)