Otx2^tm7Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm7Sia
• Name: orthodenticle homeobox 2; targeted mutation 7, Shinichi Aizawa
• MGI ID: MGI:3046841
• Synonyms: Otx2^deltaAN, Otx2^deltaAN1
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm7Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91012
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The anterior neuroectoderm (AN) enhancer region (559 bp) was replaced with a floxed neomycin-resistance gene. Southern blot confirmed recombination and quantitative RT-PCR confirmed a reduction in gene expression in mutant embryos. (J:91012)

Expression

In Mice Carrying this Mutation:	19 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:91012 Kurokawa D, et al., Regulation of Otx2 expression and its functions in mouse epiblast and anterior neuroectoderm. Development. 2004 Jul;131(14):3307-17
• All: 3 reference(s)