Mmp14^tm1Noda
Targeted Allele Detail

Summary

• Symbol: Mmp14^tm1Noda
• Name: matrix metallopeptidase 14 (membrane-inserted); targeted mutation 1, Makoto Noda
• MGI ID: MGI:3046860
• Synonyms: MT1-MMP^-, MT1-MMP KO
• Gene: Mmp14 Location: Chr14:54669055-54679913 bp, + strand Genetic Position: Chr14, 27.79 cM
• Alliance: Mmp14^tm1Noda page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91198
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The allele was created with a targeting vector replacing and disrupting exons 1-5, which encode the catlytic domain, with a gene encoding LacZ fused to a nuclear localizing signal. The PGK-gpt/neomycin-resistance gene cassette was inserted in the reverse direction, downstrem of lacZ. Northern blot analysis of homozygous mutant fibroblasts show no detectable mRNA and absence of protein was demonstrated in Western blot of mutant embryos. (J:91198, J:158339)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mmp14 Mutation: 44 strains or lines available

References

• Original: J:91198 Oh J, et al., Mutations in two matrix metalloproteinase genes, MMP-2 and MT1-MMP, are synthetic lethal in mice. Oncogene. 2004 Jun 24;23(29):5041-8
• All: 9 reference(s)