Grem1^tm1Azun
Targeted Allele Detail

Summary

• Symbol: Grem1^tm1Azun
• Name: gremlin 1, DAN family BMP antagonist; targeted mutation 1, Aimee Zuniga
• MGI ID: MGI:3046937
• Synonyms: deltaGre, Gre^deltaORF, Grem1^delta, Gremlin^-
• Gene: Grem1 Location: Chr2:113579020-113588993 bp, - strand Genetic Position: Chr2, 57.43 cM
• Alliance: Grem1^tm1Azun page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90991
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire 552 bp CDS encoded by exon 2 and 132 bp of the 3' UTR were deleted and replaced by an IRES-lacZ cassette and a loxP site flanked neomycin resistance gene cassette. In situ hybridization of heterozygous embryos show identical distribution of wild-type and mutant allele expression. (J:90991)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grem1 Mutation: 19 strains or lines available

Notes

This mutation was determined to be allelic to both Fmn1^{ld-Is(17;In2)1Gso} and Fmn1^tm1.1Zlr through complementation analyses (J:91385). Transcription was not detected in the limb bud mesenchyme of either type of compound heterozygous mutant embryo. This allele is allelic to Grem1^ld (J:91385) as well. In each case, compound heterozygous mutant mice display the mutant "limb deformity" limb phenotype.

References

• Original: J:90991 Michos O, et al., Gremlin-mediated BMP antagonism induces the epithelial-mesenchymal feedback signaling controlling metanephric kidney and limb organogenesis. Development. 2004 Jul;131(14):3401-10
• All: 10 reference(s)