Trgc1^tm1.2Dhr
Targeted Allele Detail

Summary

• Symbol: Trgc1^tm1.2Dhr
• Name: T cell receptor gamma, constant 1; targeted mutation 1.2, David H Raulet
• MGI ID: MGI:3046956
• Synonyms: 234JCgamma1^-, Cr1^-
• Gene: Trgc1 Location: Chr13:19398273-19400788 bp, + strand Genetic Position: Chr13, 6.89 cM
• Alliance: Trgc1^tm1.2Dhr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91304
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Trgc1^tm1.2Dhr involves 5 genes/genome features (Trgv4, Trgj1, Trgc1 ...) View all
• Mutation details: Transient Cre expression in Tcrg-C1^tm1.1Dhr ES cells, which already lack enhancer elements HsA and Ecgamma1, excised the floxed region containing Vgamma2, Vgamma4, Vgamma3, Jgamma1, Cgamma1. Knockout mice were identified using Southern blot and PCR analyses. In situ immuno-staining of skin sheets of mutants were nearly devoid of TCRgammadelta cells. Flow cytometric analysis also indicated absence of dendritic epidermal T cells. (J:91304)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trgc1 Mutation: 4 strains or lines available

References

• Original: J:91304 Xiong N, et al., Positive selection of dendritic epidermal gammadelta T cell precursors in the fetal thymus determines expression of skin-homing receptors. Immunity. 2004 Jul;21(1):121-31
• All: 2 reference(s)