Tnfsf13b^tm1Cleg
Targeted Allele Detail

Summary

• Symbol: Tnfsf13b^tm1Cleg
• Name: tumor necrosis factor (ligand) superfamily, member 13b; targeted mutation 1, Chris H Clegg
• MGI ID: MGI:3047300
• Synonyms: BlyS^-
• Gene: Tnfsf13b Location: Chr8:10056637-10086000 bp, + strand Genetic Position: Chr8, 4.55 cM
• Alliance: Tnfsf13b^tm1Cleg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71301
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequence beginning with codon 2 in exon 1 and spanning to the first 19 bp in intron 1 (through codon 86) was deleted and replaced with an IRES BetaGal-PolyA/MC1neo-PolyA cassette. The deleted sequence encoded the cytoplasmic and transmembrane domains. Southern blot confirmed homologous recombination and Northern blot of lungs indicated no transcript was present in mutants. ELISA showed at least 50% decreases in IgM and IgG levels in serum of mutant mice. (J:71301)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfsf13b Mutation: 30 strains or lines available

References

• Original: J:71301 Gross JA, et al., TACI-Ig neutralizes molecules critical for B cell development and autoimmune disease. impaired B cell maturation in mice lacking BLyS. Immunity. 2001 Aug;15(2):289-302
• All: 1 reference(s)