Pbx2^tm1Mlc
Targeted Allele Detail

Summary

• Symbol: Pbx2^tm1Mlc
• Name: pre B cell leukemia homeobox 2; targeted mutation 1, Michael L Cleary
• MGI ID: MGI:3047327
• Synonyms: Pbx2^-
• Gene: Pbx2 Location: Chr17:34811233-34816374 bp, + strand Genetic Position: Chr17, 18.17 cM
• Alliance: Pbx2^tm1Mlc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91079
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 248 bp portion of exon 3 and 68 bp of intron 3 were deleted and replaced with a PGK-neo cassette. Western blot of postnatal thymocytes and bone marrow cells indicated absence of protein. Western blot of fetal livers raised against a peptide mapping at the amino acid terminus detected neither full length nor truncated proteins. Northern blot also indicated no full length or truncated transcript. (J:91079)

Expression

In Mice Carrying this Mutation:

52 assay results 3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pbx2 Mutation: 14 strains or lines available

Notes

ES cell line = W9.5 (129S1/Sv-p⁺ Tyr⁺ Kitl⁺) or GS (129S6/SvEvTac).

References

• Original: J:91079 Selleri L, et al., The TALE homeodomain protein Pbx2 is not essential for development and long-term survival. Mol Cell Biol. 2004 Jun;24(12):5324-31
• All: 22 reference(s)