Myl2^{tm1(Hand1)Tana}
Targeted Allele Detail

Summary

• Symbol: Myl2^{tm1(Hand1)Tana}
• Name: myosin, light polypeptide 2, regulatory, cardiac, slow; targeted mutation 1, Makoto Tanaka
• MGI ID: MGI:3047382
• Gene: Myl2 Location: Chr5:122239014-122251535 bp, + strand Genetic Position: Chr5, Syntenic
• Alliance: Myl2^{tm1(Hand1)Tana} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91082
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using a knock in strategy, FLAG-tagged Hand1 and a floxed neo were inserted into the Myl2 locus, replacing the coding sequence. Transient cre-mediated recombination excised the neo. Southern blot confirmed recombination and immunohistochemistry using an anti-FLAG antibody detected expression in cardio myocytes of mutant animals. (J:91082)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myl2 Mutation: 22 strains or lines available

References

• Original: J:91082 Togi K, et al., Role of Hand1/eHAND in the dorso-ventral patterning and interventricular septum formation in the embryonic heart. Mol Cell Biol. 2004 Jun;24(11):4627-35
• All: 1 reference(s)