Cd79a^tm4.1Cgn
Targeted Allele Detail

Summary

• Symbol: Cd79a^tm4.1Cgn
• Name: CD79A antigen (immunoglobulin-associated alpha); targeted mutation 4.1, University of Cologne
• MGI ID: MGI:3047537
• Synonyms: Igalpha^deltaTm
• Gene: Cd79a Location: Chr7:24596922-24601283 bp, + strand Genetic Position: Chr7, 13.49 cM
• Alliance: Cd79a^tm4.1Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91091
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Crossing Cd79a^tm4Cgn mice with deleter mice expressing cre in the germline created this allele in which floxed exons 2 and 3 were excised, thus removing the extracellular membrane and transmembrane encoding sequences. FACS analysis of mutant bone marrow and spleen indicated complete lack of B cell development and T cell numbers decreased to 30-50% of wild-type controls. Mu chain was detected in the cytoplasm of pro-B cells, demonstrating VDJ recombination was functional. (J:91091)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd79a Mutation: 24 strains or lines available

References

• Original: J:91091 Kraus M, et al., Survival of resting mature B lymphocytes depends on BCR signaling via the Igalpha/beta heterodimer. Cell. 2004 Jun 11;117(6):787-800
• All: 3 reference(s)