Pax3^{tm3(Pax7)Buck}
Targeted Allele Detail

Summary

• Symbol: Pax3^{tm3(Pax7)Buck}
• Name: paired box 3; targeted mutation 3, Margaret Buckingham
• MGI ID: MGI:3047631
• Synonyms: Pax3^{DsRed(Pax7-ILZ)}
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^{tm3(Pax7)Buck} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90568
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding sequence in exon 1 and 4 kb of sequence including exons 2-4 were replaced with a floxed Dsred-FRT-puro-pA cassette, Pax7 cDNA and an FRT flanked IRESnlacZ cassette. The splice donor site was deleted to ensure that no functional Pax3 protein could be made. Southern blot confirmed recombination. (J:90568)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:90568 Relaix F, et al., Divergent functions of murine Pax3 and Pax7 in limb muscle development. Genes Dev. 2004 May 1;18(9):1088-105
• All: 1 reference(s)