Pax3^{tm3.1(Pax7)Buck}
Targeted Allele Detail

Summary

• Symbol: Pax3^{tm3.1(Pax7)Buck}
• Name: paired box 3; targeted mutation 3.1, Margaret Buckingham
• MGI ID: MGI:3047632
• Synonyms: Pax3^Pax7-ILZ
• Gene: Pax3 Location: Chr1:78077904-78173771 bp, - strand Genetic Position: Chr1, 39.79 cM
• Alliance: Pax3^{tm3.1(Pax7)Buck} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90568
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Crossing of Pax3^{tm3(Pax7)Buck} with mice expressing Cre in the germline create this allele in which the DsRed-FRT-puro-pA was excised, allowing expression of knocked in Pax7. In situ hybridization showed that Pax3 transcripts are detectable in mutant embryos, suggesting either the use of a cryptic promoter and/or alternative splicing, or leakiness of the 3' poly A sequence following the nLacZ. Immuno histochemistry indicates binding of antibodies to beta-gal in somites of mutants. (J:90568)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pax3 Mutation: 50 strains or lines available

References

• Original: J:90568 Relaix F, et al., Divergent functions of murine Pax3 and Pax7 in limb muscle development. Genes Dev. 2004 May 1;18(9):1088-105
• All: 2 reference(s)