Rab3ip^Gt(pPAT)1Sia
Gene trapped Allele Detail

Summary

• Symbol: Rab3ip^Gt(pPAT)1Sia
• Name: RAB3A interacting protein; gene trap 1, Shinichi Aizawa
• MGI ID: MGI:3047686
• Gene: Rab3ip Location: Chr10:116741685-116786361 bp, - strand Genetic Position: Chr10, 64.96 cM
• Alliance: Rab3ip^Gt(pPAT)1Sia page

Mutation origin

• Mutant Cell Line: PAT-12
• Germline Transmission: Earliest citation of germline transmission: J:29606
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector pPAT containing intron 1 and the splicing acceptor from Fyn, lacZ-pA, Amp^r, PGK-neo and the splicing donor from Fyn was inserted at the 3' end of the endogenous gene. Northern blot of mutant mice indicated no wild-type transcripts present in brain, testis or kidney, but showed evidence of fusion transcripts between the neo and the 3' end of Rab3ip. RT-PCR of mutant kidney and testis confirmed lack of wild-type transcript. (J:29606)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab3ip Mutation: 42 strains or lines available

References

• Original: J:29606 Yoshida M, et al., A new strategy of gene trapping in ES cells using 3'RACE. Transgenic Res. 1995 Jul;4(4):277-87
• All: 1 reference(s)