Xist^tm3Nbd
Targeted Allele Detail

Summary

• Symbol: Xist^tm3Nbd
• Name: inactive X specific transcripts; targeted mutation 3, Neil Brockdorff
• MGI ID: MGI:3047786
• Synonyms: SPA+neo
• Gene: Xist Location: ChrX:102503979-102526839 bp, - strand Genetic Position: ChrX, 46.15 cM, cytoband D
• Alliance: Xist^tm3Nbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85305
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An insertion of the transcriptional terminator site, SPA-MAZ4 into the region 1.1 kb upstream of the P1 start site. Also, a floxed neomycin resistance gene was inserted. The insertion was designed to disrupt any transcription between the promoter and upstream regions. RT-PCR indicates presence of ectopic sense transcription upstream of Xist in mutants. (J:85305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Xist Mutation: 8 strains or lines available

References

• Original: J:85305 Nesterova TB, et al., Skewing X chromosome choice by modulating sense transcription across the Xist locus. Genes Dev. 2003 Sep 1;17(17):2177-90
• All: 1 reference(s)