Rps12-ps1/Xist^tm1Nbd
Targeted Allele Detail

Summary

• Symbol: Rps12-ps1/Xist^tm1Nbd
• Name: ribosomal protein S12, pseudogene 1; targeted mutation 1, Neil Brockdorff
• MGI ID: MGI:3047790
• Synonyms: DeltaCJ+neo
• Gene: Rps12-ps1 Location: ChrX:102533123-102533523 bp, + strand Genetic Position: ChrX, 46.17 cM
• Alliance: Rps12-ps1/Xist^tm1Nbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85305
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A 2.5 kb sequence was deleted between 5.5 and 8 kb upstream of Xist, including Rps12-ps1 and spanning a site thought to include an upstream promoter for Xist. A floxed PGK-neo was also inserted. RT-PCR showed presence of antisense transcription upstream of Xist in mutants where none is present in wild-type. (J:85305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rps12-ps1 Mutation: 0 strains or lines available

References

• Original: J:85305 Nesterova TB, et al., Skewing X chromosome choice by modulating sense transcription across the Xist locus. Genes Dev. 2003 Sep 1;17(17):2177-90
• All: 1 reference(s)