Dnmt3b^tm5.2Enl
Targeted Allele Detail

Summary

• Symbol: Dnmt3b^tm5.2Enl
• Name: DNA methyltransferase 3B; targeted mutation 5.2, En Li
• MGI ID: MGI:3047834
• Synonyms: Dnmt3b^1lox
• Gene: Dnmt3b Location: Chr2:153491370-153529650 bp, + strand Genetic Position: Chr2, 75.79 cM, cytoband A2-A3
• Alliance: Dnmt3b^tm5.2Enl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91108
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was created by crossing Dnmt3b^tm5.1Enl with TNAP-cre mice, thereby excising the floxed segment, which includes exon 19, in the germline. This results in out-of-frame splicing of the mRNAs encoding all three active forms of the protein. (J:91108)

Expression

In Mice Carrying this Mutation:	9 assay results 7 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt3b Mutation: 52 strains or lines available

References

• Original: J:91108 Kaneda M, et al., Essential role for de novo DNA methyltransferase Dnmt3a in paternal and maternal imprinting. Nature. 2004 Jun 24;429(6994):900-3
• All: 6 reference(s)