En1^{tm1(PDGFB)Nist}
Targeted Allele Detail

Summary

• Symbol: En1^{tm1(PDGFB)Nist}
• Name: engrailed 1; targeted mutation 1, Monica Nister
• MGI ID: MGI:3047856
• Synonyms: En1-PDGFB-IRES-lacZ
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^{tm1(PDGFB)Nist} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90898
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Human PDGFB, as well as an IRES-lacZ gene and floxed neo replaced some 5' sequence of the coding region. Southern blot and PCR confirmed incorporation of PDGFB. In situ hybridization using human PDGFB also confirmed expression of the knock in gene. (J:90898)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:90898 Andrae J, et al., Forced expression of platelet-derived growth factor B in the mouse cerebellar primordium changes cell migration during midline fusion and causes cerebellar ectopia. Mol Cell Neurosci. 2004 Jun;26(2):308-21
• All: 1 reference(s)