Fzd6^tm1Nat
Targeted Allele Detail

Summary

• Symbol: Fzd6^tm1Nat
• Name: frizzled class receptor 6; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:3050103
• Synonyms: Fz6-
• Gene: Fzd6 Location: Chr15:38869673-38901587 bp, + strand
• Alliance: Fzd6^tm1Nat page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91412
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The nLacZ gene and a loxP site flanked neomycin resistance gene cassette were inserted in place of codons 1-125, which encode the signal sequence and most of the cystein-rich ligand-binding domain. PCR confirmed integration of neo and the absence of endogenous sequence and X-gal staining of mutant skin showed that the gene was expressed. Western blot against the C-terminal 17 amino acids of the endogenous gene demonstrated its absence in mutant skin. The floxed neo was later removed through crossing with germ-line Cre-expressing mice. (J:91412)

Expression

In Mice Carrying this Mutation:	59 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fzd6 Mutation: 42 strains or lines available

References

• Original: J:91412 Guo N, et al., Frizzled6 controls hair patterning in mice. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9277-81
• All: 27 reference(s)