Kcnma1^tm1Ruth
Targeted Allele Detail

Summary

• Symbol: Kcnma1^tm1Ruth
• Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1; targeted mutation 1, P Ruth
• MGI ID: MGI:3050114
• Synonyms: BK^-, BKalpha^-, BK-KO, BK L1
• Gene: Kcnma1 Location: Chr14:23342356-24055173 bp, - strand Genetic Position: Chr14, 12.92 cM
• Alliance: Kcnma1^tm1Ruth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91427
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A single loxp site was inserted upstream of the exon encoding the pore-forming alpha subunit, and a floxed neo-TK cassette downstream. Transient cre-expression excised the floxed region including the exon and neo-TK. Southern blot and PCR confirmed recombination and Western blot of brain membrane proteins from mutants indicated absence of the C-terminus of the alpha subunit. (J:91427)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnma1 Mutation: 101 strains or lines available

References

• Original: J:91427 Sausbier M, et al., Cerebellar ataxia and Purkinje cell dysfunction caused by Ca2+-activated K+ channel deficiency. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9474-8
• All: 30 reference(s)