E4f1^tm1Pisc
Targeted Allele Detail

Summary

• Symbol: E4f1^tm1Pisc
• Name: E4F transcription factor 1; targeted mutation 1, Piotr Sicinski
• MGI ID: MGI:3050154
• Synonyms: E4F^-
• Gene: E4f1 Location: Chr17:24662752-24674366 bp, - strand Genetic Position: Chr17, 12.39 cM
• Alliance: E4f1^tm1Pisc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91435
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3-14 were replaced with a puromycin resistance gene. The removed sequence encodes amino acids 104-784, including all the C2H2 zinc-finger domains responsible for DNA binding and functional activities. Southern blotting confirmed recombination. (J:91435)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any E4f1 Mutation: 38 strains or lines available

References

• Original: J:91435 Le Cam L, et al., The E4F protein is required for mitotic progression during embryonic cell cycles. Mol Cell Biol. 2004 Jul;24(14):6467-75
• All: 6 reference(s)