Kcnk4^tm1Lzd
Targeted Allele Detail

Summary

• Symbol: Kcnk4^tm1Lzd
• Name: potassium channel, subfamily K, member 4; targeted mutation 1, Michel Lazdunski
• MGI ID: MGI:3050222
• Synonyms: TRAAK^-
• Gene: Kcnk4 Location: Chr19:6903030-6912261 bp, - strand Genetic Position: Chr19, 5.08 cM
• Alliance: Kcnk4^tm1Lzd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91531
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 100 bp of exon 2, encoding most of the first transmembrane domain, was replaced by an IRES-geo-neo cassette. The insertion was 54 bp after the start codon of the endogenous coding sequence, and the presence of stop codons in three reading frames upstream of the IRES-geo-neo allowed interruption of translation of the endogenous mRNA 18 amino acids after the beginning. Southern blot confirmed recombination and Western blot indicated lack of wild-type protein in mutant brains. (J:91531)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnk4 Mutation: 21 strains or lines available

References

• Original: J:91531 Heurteaux C, et al., TREK-1, a K(+) channel involved in neuroprotection and general anesthesia. EMBO J. 2004 Jul 7;23(13):2684-95
• All: 10 reference(s)