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Slc4a2tm1Ges
Targeted Allele Detail
Summary
Symbol: Slc4a2tm1Ges
Name: solute carrier family 4 (anion exchanger), member 2; targeted mutation 1, Gary E Shull
MGI ID: MGI:3050237
Synonyms: AE2-
Gene: Slc4a2  Location: Chr5:24628834-24645945 bp, + strand  Genetic Position: Chr5, 11.74 cM
Alliance: Slc4a2tm1Ges page
Growth retardation and lack of incisor tooth eruption in Slc4a2tm1Ges/Slc4a2tm1Ges mice

Show the 6 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:91617
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA neo cassette replaced exons 14-17. Transcripts were expected to utilize the neo polyA, thereby lacking codons 703-1237, which encode the transmembrane domains. Northern blot using probes of sequences 3' to the neo showed that wild-type mRNAs were ablated in mutants, whereas probes of sequences 5' to the neo indicated presence of transcripts consistent in size with two splice variants. An immunofluorescence assay using an antibody that recognizes the C terminus confirmed protein disruption. (J:91617)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 3 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Slc4a2 Mutation:  53 strains or lines available
References
Original:  J:91617 Gawenis LR, et al., Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric. J Biol Chem. 2004 Jul 16;279(29):30531-9
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory