Slc4a2^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Slc4a2^tm1Ges
• Name: solute carrier family 4 (anion exchanger), member 2; targeted mutation 1, Gary E Shull
• MGI ID: MGI:3050237
• Synonyms: AE2^-
• Gene: Slc4a2 Location: Chr5:24628834-24645945 bp, + strand Genetic Position: Chr5, 11.74 cM
• Alliance: Slc4a2^tm1Ges page

Growth retardation and lack of incisor tooth eruption in Slc4a2^tm1Ges/Slc4a2^tm1Ges mice

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91617
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neo cassette replaced exons 14-17. Transcripts were expected to utilize the neo polyA, thereby lacking codons 703-1237, which encode the transmembrane domains. Northern blot using probes of sequences 3' to the neo showed that wild-type mRNAs were ablated in mutants, whereas probes of sequences 5' to the neo indicated presence of transcripts consistent in size with two splice variants. An immunofluorescence assay using an antibody that recognizes the C terminus confirmed protein disruption. (J:91617)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc4a2 Mutation: 53 strains or lines available

References

• Original: J:91617 Gawenis LR, et al., Mice with a targeted disruption of the AE2 Cl-/HCO3- exchanger are achlorhydric. J Biol Chem. 2004 Jul 16;279(29):30531-9
• All: 10 reference(s)