A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}
Transgenic Allele Detail

Summary

• Symbol: A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}
• Name: APOBEC1 complementation factor; transgene insertion 1, Jeffery D Molkentin
• MGI ID: MGI:3050453
• Synonyms: alphaMHC-Cre-Mer-Cre, alpha-MHC-MerCreMer, CM-MCM, MCM, mer, mER-CRE-mER, MerCreMer, Myh6-MerCreMer, Myh6:MerCreMer, Tg(Myh6-cre/Esr1)1Jmk
• Gene: A1cf Location: Chr19:31846164-31926395 bp, + strand Genetic Position: Chr19, 26.58 cM, cytoband C3
• Alliance: A1cf^{Tg(Myh6-cre/Esr1*)1Jmk} page

The Med1^tm2Jkr/Med1^tm2Jkr A1cf^{Tg(Myh6-cre/Esr1*)1Jmk}/A1cf⁺ mouse heart is flaccid and flabby after tamoxifen induction.

Show the 6 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• A1cf^{Tg(Myh6-cre/Esr1*)1Jmk} expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh6 (MGI:97255)

• Mutation details: This transgene expresses a cre recombinase/ mutant estrogen receptor ligand binding domain fusion protein under the control of a mouse cardiac-specific alpha-myosin heavy chain promoter. Expression was detected in the juvenile and adult heart, but the protein is inactive until induced with tamoxifen. Integration of the transgene in to Chr. 19 deleted a 19,500 bp fragment including the promoter, exon 1, and part of intron 1 of the A1cf gene. Western analysis of liver from homozygous transgenic mice does not detect A1cf protein. (J:82027, J:231727)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myh6 (mouse) Summary of all recombinase alleles driven by Myh6. Comparative matrix view of recombinase activities Alliance mouse Myh6 gene page

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any A1cf Mutation: 39 strains or lines available

References

• Original: J:82027 Sohal DS, et al., Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using a tamoxifen-inducible Cre protein. Circ Res. 2001 Jul 6;89(1):20-5
• All: 496 reference(s)