Rasd2^tm1Rdl
Targeted Allele Detail

Summary

• Symbol: Rasd2^tm1Rdl
• Name: RASD family, member 2; targeted mutation 1, Roberto Di Lauro
• MGI ID: MGI:3050759
• Synonyms: Rhes^-
• Gene: Rasd2 Location: Chr8:75940572-75950741 bp, + strand Genetic Position: Chr8, 35.61 cM
• Alliance: Rasd2^tm1Rdl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91597
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was used to insert a stop codon in each frame immediately downstream of the endogenous translational start codon. An IRES-EGFP was placed downstream of the endogenous regulatory sequences and a floxed PGKneo were also inserted. Southern blot confirmed recombination. In situ hybridization showed that mRNA was barely detectable in the striatal region of mutant brains. In situ hybridization using an antisense riboprobe of EGFP shows that EGFP mRNA was detected in mutants. Western blotting of striatum protein extracts showed putatively nonspecific bands that were present in both wild-type and knockouts. Therefore, the mutated allele produces no detectable Rasd2 protein. (J:91597)

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rasd2 Mutation: 15 strains or lines available

References

• Original: J:91597 Spano D, et al., Rhes is involved in striatal function. Mol Cell Biol. 2004 Jul;24(13):5788-96
• All: 13 reference(s)